After a successful assay, the next steps in the experiment require re-streaking colonies onto other plates. After the colonies grow, then we will perform a mini-prep. The purpose of a mini-prep is to extract the DNA plasmid from yeast and E-coli cells. During this experimental step, we will be extracting the plasmids from yeast cells. I also think that this might be a good stopping point in the semester. I will resume this experiment this upcoming fall semester.
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