This week in the lab, I went through all 30 plates to determine which colonies were abundantly grown on our -T,L plates that had added 3AT and Bexarotene. I was able to pick off 54 colonies to use in our next phase of the experiment. The process I completed this week was streaking the 54 colonies onto -T,L plates to see which colonies will regrow. After that step, I will perform a Lac Z assay on the remaining colonies. I am a fan of the Lac Z assay because that means playing with...I mean "utilizing" liquid nitrogen during the experiment.
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