I love Liquid Nitrogen
I am getting to the point in my lab project where I get to do an assay again. I remember liking this part in the experiment because I was able to use liquid nitrogen. This procedure is a little longer than an alternative procedure that we found but I like using LN so much that it's worth the extra time in the lab. The point of this procedure is to allow us to identify if a colony of yeast cells express the β-galactosidase enzyme expression. When β-galactosidase bind to the X-Gal, it cleave the molecule which then makes a blue pigment, which is very evident. This activity indicated that there is the LacZ gene was transcribed and translated.
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