Transformation of DNA into E-coliAfter the X-Gal Assay was performed and we saw the presence of Lac Z, we isolated the DNA from three colonies by performing a Zymoprep for a liquid culture. After the zymoprep was complete, we tested the purity of DNA before we transformed it into E-coli cells. The purpose of transforming the DNA into E-coli cells is to try and isolate the prey from the bait. The transformed cells will be left to incubate for 4 days on LB Amp plates containing Leucine.
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