Yeast Transformation
This week in the lab, we successfully transformed the Bait and Prey on minus Tryptophan, Leucine, and Histidine plates with 50mM of 3AT. After a growth period of about a week, each plate was analyzed and sixty-five colonies were restreaked onto minus Tryptophan, Leucine, and Histidine plates with 50mM of 3AT. The plates will be given a growth period of another week. The colonies that are able to grow on these plates will then be assayed and then sent for DNA sequencing.
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