Last week ended with a restreak of yeast colonies onto master plates with minus Tryptophan, Leucine, Histidine with 50mM of 3AT. After letting the plates grow for about a week, we were able to perform an assay on 3 colonies. The assay results revealed that the yeast cells expressed the β-galactosidase enzyme. When X-Gal adheres to the β-galactosidase, a blue pigment will be visible. This activity indicated that there is a LacZ gene present. In order to verify the results, the three samples will be sent out for sequencing. My favorite part about this assay is that it requires the use of Liquid Nitrogen, and let's just say that it was a very fun day of "science-ing" in the lab!
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