Additionally, the creosote plate samples from the White Tank Mountain area were continued to be further isolated with the goal of obtaining pure culture samples.
Thursday, October 26, 2017
9: Creosote Bacteria Identification Continued
Continued:Creosote Bacteria Identification for "PC Sample" and "White Tank Mountain Samples"
This week I continued to perform two more tests on the creosote sample that was taken on the Phoenix College campus. The first test I performed was Mannitol Salt Agar (MSA) which is used to identify if a bacteria can tolerate a high saline concentration. The second test performed this week was called MacConkey Agar (MAC) which is selective for gram-negative bacteria. The inoculated plates were placed in the incubator at 37 degrees Celsius and results will be recorded by the end of the school week.
Additionally, the creosote plate samples from the White Tank Mountain area were continued to be further isolated with the goal of obtaining pure culture samples.
Additionally, the creosote plate samples from the White Tank Mountain area were continued to be further isolated with the goal of obtaining pure culture samples.
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Hey Sam, it’s very exciting to see you move along and make progress in your project. Not to mention that you’re learning new material and procedures that can potentially help you in future research! The tests you ran seem quite interesting because I have never heard of them or performed them before. Great job on moving forward! :)
ReplyDeleteHey, Sam welcome to the Microbiology team. Once you start using test medias (selective or differential), you can consider yourself a part of the team ha-ha. You also mentioned you used both MAC and MSA plates, are there any results you’re expecting from your creosote sample? Also, I like the image you provided. It’s good to throw in some humor now and then.
ReplyDeleteThank you! Funny story about the MAC tests. When I took out all 9 plate samples, I saw no growth on any of them....of course, I had a hunch I did something wrong. It turns out I wasn't letting the reusable inoculating loop cool down enough after sterilizing it, so I was totally killing my own bacteria samples as I was inoculating them onto the MAC plates. It set me back in my schedule but created a pretty funny memory as well. As funny as it was, I learned I need to wait longer in between the sterilization process and inoculation process. So, when I redid the MAC tests this morning, I used the timer to wait 15 secs after sterilizing the inoculating loop. I also now have an even larger preference for the disposable inoculating loops.
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