Whelp, that's Research.....Whelp, the replica plating didn't turn out as we expected, so we decided to just perform a transformation on a media plate with the 3AT already included. After, I made a new batch of agar media, an overnight culture of yeast cells was started. The next morning, we noticed that the cells weren't growing at the needed rate. We think the cells weren't really growing because of the age of the original yeast culture. Then, it was decided to go back and redo the bait transformation, but I am okay with that because it means more practice for me. The week ended with the bait transformation on a -Tryp media plate. If the transformation is successful, we will see cell growth on the -Tryp plate, and then start an overnight culture in preparation of transforming the cells with the DNA library.
Wowzers, I'm glad you are keeping your chin up throughout this process. It sounds quite complicated if I'm honest. I've been keeping up with your blog because your project is interesting but half the time I'm not sure what you all are doing just because I am not well versed in biology. But with your yeast cultures and tests you are running are you mostly independent in your endeavors and tests or is the person you are working under telling you what tests and procedures to run?
ReplyDeleteOh my goodness! It's good to hear other people having these super stop and go and go back and stop and go LOL I'm still learning to cope with how it feels to lose research time because something absolutely out of your control mucks up your stuff.
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