That Yeast is PosioneddddThis week in the lab, we moved onto the step where we were going to "poison" our yeast cells. When we initially did the first transformation, we noticed that large colonies were forming on the plates which indicated that the bait was still causing transcription and producing histidine. With that observation, we decided that we needed to figure out which concentration of 3AT we could get our cells to grow in without the bait causing the histidine production during transcription. With that said, I made five different concentrated solutions of 3AT and made sample transformations to see which concentration will work best with our needs. Hopefully, the next time I go into the lab, I will be able to see which concentration worked.
Hey Sam! I always read your blog and never end up commenting. I went to the Women of Color in STEM Conference this weekend and Dr Nicol Turner-Lee spoke about how she was trained in sociology, and it made me think of you. She's currently working at the Brookings Institution and involved in what sounds like a whole slew of things including policy making and writing a book about the people who are digitally invisible particularly due to a lack of access to internet for various reasons. I can hardly wait to buy it. She seems absolutely wonderful and her research is fascinating. Anyway, because I know that you got your bachelors degree in sociology, I was just wondering if you were planning to use that in any of your future plans and if so how or if not why? I'm very interested in sociology, and I keep meaning to ask you and I always forget.
ReplyDelete