Today in the lab, I focused on trying to do some trouble-shooting in order to extract the third and final plasmid to be sent for sequencing. My PI is thinking that there is possibly a mutation in this sample that is causing cells to produce its own LacZ and histindine. For this reason, we believe that this is why we were seeing blue cells when we performed the Xgal Assay awhile back. So, I am going to try two more transformation with different DNA concentrations to see if we get sufficient results this time. If not, we are going with the mutation idea and sending our other two samples for sequencing and calling it a day.
Thursday, February 14, 2019
S4 30: Trouble Shooting
Today in the lab, I focused on trying to do some trouble-shooting in order to extract the third and final plasmid to be sent for sequencing. My PI is thinking that there is possibly a mutation in this sample that is causing cells to produce its own LacZ and histindine. For this reason, we believe that this is why we were seeing blue cells when we performed the Xgal Assay awhile back. So, I am going to try two more transformation with different DNA concentrations to see if we get sufficient results this time. If not, we are going with the mutation idea and sending our other two samples for sequencing and calling it a day.
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