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This past week, I focused on testing another an alternative X-gal assay on yeast cells. This was the second alternative procedure that I had tried and it was successful. I had some extra time in the lab before the semester comes to a close, so my research mentor suggested that I try transforming the other two DNA samples again. The rationale for attempting a second transformation was because they previously tested positive for LacZ. Going back to the DNA sample that we were able to extract, I performed a miniprep on the bacteria cells to increase our plasmid yield. Hopefully, I will be able to have a successful DNA plasmid extraction from the other two samples and all three DNA samples can be sent for sequencing.
Aww, the last few science classes that I had with labs that involved DNA were total fails, and those labs were usually the ones that I was most excited about. It's good to know that should I pursue it, I could have the opportunity to bang them out until I become really good at the procedure. You seem like you're really getting comfortable over there. I'm so happy for you!
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