Thursday, November 9, 2017

11: Creosote Bacteria Identification for White Tank Mountain Samples Continued

Gram positive result from Plate 1 Colony 6

Continued:Creosote Bacteria Identification for "White Tank Mountain Samples"

The hunt for the unknown bacteria identification continues! This week I came closer to identifying a portion of the 23 unknown bacteria samples. I am currently focusing on Plate 1 (Colonies 1-7) and am performing a range of tests. I was able to identify the cell morphologies and cell wall types. Of the samples tested, they all are gram positive. I then compared the gram stain results with results from the MacConkey tests I performed on the "Plate 1" samples. The MacConkey tests confirmed that the samples are gram positive. Additionally, I ran Glucose Fermentation tests, Trypone Tests, and Sims Citrate tests on the samples and will document the results when they come out of the room temperature incubator after the weekend. I plan to continue to run the remainder of tests and indicate the results on an excel file that I have created.


2 comments:

  1. Hey, Sammy, having 23 unknown bacteria must be exciting. I’m surprised Plate 1 has only G+ bacteria only. Do you have any reasons why that turned out like that? Did you look up anything online as to why G+ bacteria could be favorable to creosote? If you don’t mind me asking (maybe you’ve said it somewhere on your blog), can you tell me what your hypothesis is?

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  2. Hi Sam, you’ve been streaking plates and running all these tests like a pro. Eventually, this hard work will pay off because you’ll be able to identify your unknowns! It’s exciting and considering the fact that I’m also using your bacteria, it’s nice to see what it looks like. Good job and keep up the great work :)

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