S4 34: Y2H Website

Y2H Website
Last week, I worked on starting the Yeast Two hybrid experiment by completing a bait transformation into yeast cells. I let the cells incubate and had a successful transformation. The next step will be to transform the prey plasmid with the bexarotene compound. However, this week I have spent my time working on the Yeast Two Hybrid website with my research mentor. The goal of the website is to have a process where students and other professors can access and learn about the Yeast Two Hybrid procedure. My favorite aspect of the website is the flow chart that we are working on so that people can have step by step guidance as they perform their own Yeast Two Hybrid screens.

S4 33: Next Phase of the Project

https://pubchem.ncbi.nlm.nih.gov/compound/Bexarotene

Next Phase
The next phase in the project includes beginning the Yeast 2 Hybrid experiment again using the same DNA prey library that we made last semester. However, this go around we are going to add a bexarotene rexinoid to the bait and see if it promotes the same type of protein binding or additional protein binding. I am looking forward to repeating the experiment because it will allow me to practice the Y2H processes again. I will also get to refine my research notes on the procedures which helps with the Y2H website that my research mentor and I are working on.

S4 32: Sequencing Update

                                                                                   

Sequencing Update

This week in the lab, we got the results back from our plasmids that we sent to get genetically sequenced. They came back without any problems so I guess my dilutions were right. I am glad! It's a funny reminder of how I need to be more confident and trusting of myself in the lab setting. Out of the four samples we sent to be sequenced we got matches from the Blast data base. The next steps of our project are to repeat the yeast two hybrid with added concentrations of Bexarotene and see if we get the same type of RXR binding as we did we the current sample. But for now, I get to enjoy spring break first.